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When considering the causes of biological failure of a water body
            (Figure 1.6, Box 1), we generally take four things into account:






               Our professional         The existing pressures      The tools and            Existing biological data
               knowledge of the         in the water body and       methods that we          (including external
               response of biology      wider catchment,            use to diagnose the      data), its trends and its
               to pressures             taking local knowledge      causes of biological     statistical associations
                                        into account                pressure                 with pressures





            These enable us to identify failures to meet objectives and
            to infer the causes of failures (Fig 1.6, Box 2). When we do
            this, we also assess whether the level of evidence linking the
            pressure with the biological failure is sufficient to support
            action (Fig 1.6, Box 3) as set out in the guidance on ‘Levels of
            evidence for completing investigations and selecting measures’.
            Where there is sufficient evidence, the next steps, eg an
            investigation to determine the source of the pressure and/or
            implementing measures, can proceed. The pressure might be
            high phosphate from agriculture and the proposed action may
            be revised guidance on fertiliser application. However, if the
            level of evidence does not support action, ie there is insufficient
            evidence linking the pressure to the biological impact to justify
            action, then we conclude that we can’t infer the cause of the
            failure with sufficient confidence (Fig 1.6, Box 6).
            If we can’t infer the likely cause of failure based on the initial
            assessment (Fig 1.6, Box 2), then we need to judge if the current
            data is adequate for the application of the diagnostic tools or to
            apply professional judgement (Fig 1.6, Box 4). Where the data is
            inadequate, we then gather more or different data (Fig 1.6, Box
            5). If the data is sufficient to apply the tools but we can’t infer
            what causes the failure (Fig 1.6, Box 6) our next step depends on
            the level of certainty associated with the cause of failure.

            Where we are uncertain about what causes the failure, we
            need to explore the situation by gathering and assessing more
            extensive data (Fig 1.6, Box 7). This might include increasing the
            number of biological elements sampled at the water body, and/
            or it may include collecting more data on pressures.

            Where we have a good idea of what causes the failure
            (Fig 1.6, Box 8), we would normally intensify monitoring, focusing
            on the biological elements most likely to be affected by the
            pressure in question (Fig 1.6, Box 9). For example, where the
            suspected pressure is flow, invertebrate analysis might be taken
            to species (rather than family) level to improve the strength of
            evidence linking the pressure to the failure. Occasionally, we
            might undertake an experimental application of a measure to
            reduce the pressure to demonstrate if this improves the biology
            (ie ‘Adaptive Management’) (Fig 1.6, Box 10).


            Our knowledge of biological responses to pressures and our
            diagnostic toolkit will improve further as we repeat this process
            over time and at multiple water bodies.


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