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5.7.1  Network of reference sites



            In the AQEM project, criteria for the selection of reference condition sites were described as follows:

              A reference stream should fulfil all requirements necessary       Physical and chemical conditions
              to allow a completely undisturbed fauna to develop and   There should be:
              establish itself. Therefore, reference sites should not only   •  no point sources of pollution or nutrient input affecting
              be characterised by clean water but also by undisturbed   the site
              stream morphology and near-natural catchment     •  no point sources of eutrophication affecting the site
              characteristics. Though it is impossible to find sites in   •  no sign of diffuse inputs or factors which suggest that
              such a pristine condition for many stream types, AQEM   diffuse inputs are to be expected
              has defined the following criteria, which should be met by
              realistic reference sites:                       •  normal background levels of nutrient and chemical
                                                                 base load, which reflect a specific catchment area
                                                               •  no sign of acidification
                Basic statements                               •  no liming activities
                                                               •  no impairments due to physical conditions; in particular,
            •  The reference condition must be practical, achievable    thermal conditions must be close to natural
               within a river basin and reasonable.
                                                               •  no local impairments due to chemical conditions;
            •  A reference site, or process for determining it, must hold    in particular, no known point-sources of significant
               or consider important aspects of natural conditions.   pollution, all the while considering the near-natural
            •  The reference conditions must reflect only minimal   pollution capacity of the water body
               anthropogenic disturbance.                      •  no sign of salinity.
                Land use practices in the catchment area           Biological conditions

            •  In most countries there is anthropogenic influence within   There must not be any:
               the catchment area. Therefore, the degree of urbanisation,
               agriculture and silviculture (forestry) should be as low as   •  significant impairment of the indigenous biota by
               possible for a site to serve as a reference site. No absolute   introduction of fish, crustaceans, mussels or any
               minimum or maximum values have been set for the defining   other kind of plants and animals
               reference conditions (eg % arable land use, % native forest);   •  significant impairment of the indigenous biota by
               instead, the least-influenced sites with the most natural   fish farming.
               vegetation are to be chosen.
                                                               In many cases, particularly in lowland stream types or larger
                River channel and habitats                     rivers, no reference sites meeting the above criteria are
                                                               available. For these stream types, the best available sites,
            •  The reference site floodplain should not be cultivated.
               If possible, it should be covered with natural climax   which meet most of the criteria, should only be a starting
               vegetation and/or unmanaged forest.             point. The description of these reference communities
                                                               should be supplemented by an evaluation of historical data
            •  Coarse woody debris must not be removed (minimum
               demand: presence of coarse woody debris).       and possibly the biotic composition of comparable stream
                                                               types, eg streams of a similar size but located in a different
            •  Stream bottoms and stream margins must not be fixed.
                                                               ecoregion (AQEM consortium, 2002).
            •  Preferably, there should be no migration barriers (affecting
               the sediment transport and/or the biota of the sampling site).
            •  Only moderate influence due to flood protection measures   So, reference values should be based on information
               can be accepted.                                  obtained from sites at which the quality element
                Riparian vegetation and floodplain               concerned is in reference condition (ie at high status).
                                                                 UK TAG state that this does not mean that at these sites
            •  Natural riparian vegetation and floodplain conditions must   the quality element will be entirely unaffected by human
               be retained, making lateral connectivity between the stream   activities. However, it does mean that alterations to it
               and its floodplain possible; depending on the stream type,   are expected to be minor. There are relatively few sites
               the riparian buffer zone should be greater or equal to 3x   at which all quality elements are in reference condition
               channel width.
                                                                 and from which data suitable for establishing reference
                Hydrologic conditions and regulation             values are available. Consequently, reference values
                                                                 can be derived from sites at which the quality element
            •  No alterations of the natural hydrograph and discharge   concerned is estimated to be in its reference condition
               regime should occur.
                                                                 but other elements at the sites may not be so.
            •  There should be no, or only minor upstream impoundments,
               reservoirs, weirs and reservoirs retaining sediment; no effect
               on the biota of the sampling site should be recognisable.
            •  There should be no effective hydrological alterations such
               as water diversion, abstraction or pulse releases.

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